To measure the amount of protein present in samples.This gives the researcher confidence that differences in the protein of interest are not due to errors while loading the gel. To check that all lanes in the gel contain the same amount of sample.Loading controls are antibodies to housekeeping proteins, or proteins that are expressed at equivalent levels in almost all tissues and cells. This is to check for non-specific binding and false positive results. If you still have difficulty finding a suitable control, we recommend doing a quick literature search on PubMed to see which tissues and cells express the protein of interest.Ī lysate from a cell line or tissue sample known not to express the protein you are detecting.This will usually provide you with relative levels of expression in various tissues. Check the GeneCards entry for the protein.These can also be considered suitable positive controls. These databases will often have a list of tissues that the protein is expressed in. Try looking at the Swiss-Prot or Omnigene database links on the datasheet.Any tissues, cells or lysates that have been used successfully by these customers can be considered a suitable positive control. Check to see if there are any Abreviews for the antibody.Not all the datasheets will have a suggested suitable control, and we recommend the following in these circumstances: Always ensure the tissue or cell line you use is from a tested species. We recommend you check the antibody datasheet, which will often provide a suggested positive control. It will verify that any negative results are valid. A positive result from the positive control, even if the samples are negative, will indicate the procedure is optimized and working. A positive control lysate is a lysate from a cell line or tissue sample known to express the protein you are detecting.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |